Bacteriology Lab

The major activities of the Kimron Veterinary Institute Brucellosis laboratory

The brucellosis laboratory of the Kimron Veterinary Institute was designated a National Reference Laboratory for Brucellosis in the early 1980s. In the 1990s it was recognized as an OIE Reference Laboratory for Brucellosis and very recently the seal of recognition was added by FAO as a reference laboratory for this organization, as well.

Whereas the laboratory resides in the Kimron Veterinary Institute constituting a small section of the veterinary services in the Ministry of Agriculture, its designation as a national reference laboratory was assigned by the Ministry of Health addressing human brucellosis. As such, all Israeli human isolates are sent to our laboratory for confirmation by biotyping, providing a basis for determining the epidemiology of the disease in Israel. Similarly, as part of the veterinary services, we are involved in the diagnosis of animal brucellosis in Israel and extend our expertise in this field globally assisting with diagnosis policy making for controlling the disease amongst small ruminants, cattle and camels.

Academically we participate in joint international research projects supported throughout the years by TAHRP, CDR, GIF, BARD and EMIDA covering institutes and partnerships with Egypt, Jordan, the Palestinian Authority, Kazakhstan, Germany, USA, Italy and Greece. The fields of research featured control practices including vaccination, serology and bacteriology in addition to basic science studies.

Select publications from our laboratory are listed below:
Articles published in peer reviewed journals (39 in total)

Bachrach, G., Banai, M., Fishman, Y. and Bercovier, H. (1997).
DTH activity of the Brucella L7/L12 ribosomal protein depends on posttranslational modification.
Infect. Immun. 65: 267-271.

Bardenstein, S., Mandelboim, M., Ficht, T.A., Baum, M., and Banai, M. (2002).
Identification of the Brucella melitensis vaccine strain Rev.1 in animals and humans in Israel by PCR analysis of the PstI site polymorphism of its omp2 gene. 
J. Clin. Microbiol. 40: 1475 – 1480.

Karplus, R., Ramlawi, A., Banai M., and Maayan, S. (2007).
The use of ELISA in a seroprevalence study of Brucella antibodies in West Bank Palestinian women of childbearing age.
Int. J. Infect. Dis. 11: 367 – 368 (Letter to the editors).

Friedman, B.-C., Banai, M., Friedman, B., Neheman, A., and Ephros, M. (2009). Testicular nodules in a 16-year-old boy.
Ped. Inf. Dis. J. 28: 168 – 172.
Liebes, Y., Marks, R.S., and Banai, M. (2009).
Chemiluminescent optical fiber immunosensor detection of Brucella cells presenting smooth-A antigen.
Sensors and Actuators B: Chemical 140 (2): 568 - 576.

Liebes, Y., Amir, L., Marks, R. S. and Banai, M. (2009).
Immobilization strategies of Brucella particles on optical fibers for use in chemiluminescence immunosensors.
Talanta 80 (1): 338 – 345.

Bardenstein, S., Strada, V., and Banai, M. (2010).
Brucella: A fastidious bacteria but a virulent pathogen (Editorial). 
Vet. J. 184: 126-127

Meltzer, E., Sidi, Y., Smolen, G., Banai, M., Bardenstein, S., Schwartz, E. (2010).
Sexually transmitted brucellosis in humans.
Clin. Inf. Dis. 51(2): e12-e15.

Banai, M., He, Y. 2012.
Systems biology and bioinformatics help decipher Brucella antigens involved in clinical manifestation of the disease
Front Cell Inf Microbio. 2:34. doi: 10.3389/fcimb.2012.00034

Articles published in book chapters (12 in total)

Banai, M. (2002).
Control of small ruminant brucellosis by use of Brucella melitensis Rev.1 vaccine: laboratory aspects and field applications.
Vet. Microbiol. 90, Issues 1 – 4 (A special issue on brucellosis, Guest Editors: S.M. Halling and S.M. Boyle), pp. 497 – 519.

Corbel, M. J., and Banai, M. (2005).
Genus 1. Brucella Meyer and Shaw 1920, 173 AL.
In: Bergey's Manual of Systematic Bacteriology 2nd Ed. Vol. 2, The Proteobacteria. Brenner DJ, Krieg NR, Staley JT (Eds.) pp 370-386. Springer, New York.

Bardenstein S. and Banai M. (2010).
Brucella species synchronize their life cycle to the gestation cycle of their ruminant hosts.
In: Avigdor Shafferman, Arie Ordentlich, Baruch Velan (eds.). The Challenge of Highly Pathogenic Microorganisms - Mechanisms of Virulence and Novel Medical Countermeasures.  pp. 135-146, Springer.